Genetics laboratory

The genetics laboratory was established in 1998 at the Agricultural Institute of Slovenia at the Crop and Seed Science Department. Today, the genetics laboratory is part of the Group for plant genetics and breeding at Crop Science Department (ORP). Within the laboratory, we also have the Laboratory for tissue cultures and the Laboratory for ecophysiology and crop evaluation.

The facilities of the genetics laboratory enable us to perform a wide range of analyses in the field of molecular biology, (population) genetics, genomics, DNA markers, NGS-based applications, transcriptomics, and metabolomics of plants, plant pathogens and animals. Laboratory is accredited under the standard ISO9001 and for some methods under the standard ISO17025 as well.

Major analytical research facilities

• tissueLyser, (Qiagen),
• automated system for preparing reagent mixtures QIAgility (Qiagen),
• flow cytometer CyFlow (Partec/Sysmex),
• automated system for the extraction of nucleic acids (KingFisher and MagMax, Thermo Fisher Scientific),
• QIAxcel Advanced capillary electrophoresis (Qiagen),
• analyzer of nucleic acids and proteins (Agilent),
• various types of cyclic thermostats (ABI Veriti, ABI Veriti Pro, SureCycler Agilent, ABI9700),
• fluorometer (Qubit 3.0),
• heat shaker (for macroarray, GeneTop Biotech),
• horizontal electrophoresis with a gel imaging system (Biometra, GeneGenius),
• vertical electrophoresis system (Biometra),
• NanoDrop One Microvolume UV-Vis Spectrophotometer (Thermo) (VRO package 22),
• QuantStudio Absolute Q Digital PCR System (Applied Biosystems) (VRO package 22),
• QuantStudio 5 Real-Time PCR System (Applied Biosystems) (VRO package 22) and Real time PCR ABI7500 with fast and HRM upgrade,
• additional equipment available on CFGBC/MF (active link)and BF (active link) for sharing with Agricultural institute of Slovenia as a member of the consortium.

Research related to the work in the genetics laboratory is carried out at the Institute of Agriculture within the three programme groups Agrobiodiversity, Sustainable Agriculture and Next Generation Agriculture.

Contact persons