The control of root-knot nematodes (Meloidogyne spp.) is extremely difficult. They can parasitise numerous plant species; in addition to many herbaceous species, they also attack a large number of woody plants. As a result, crop rotation is largely ineffective for containing them. In Slovenia, only a few chemical plant protection products (nematocides) are available, making it difficult to control these pests. With the aim of developing new effective and environmentally friendly approaches to control root-knot nematodes, we focus our research on the development of alternative approaches to control plant-parasitic nematodes, such as biofumigation (control of soil pests through the use of volatile chemical substances, e.g. glucosinolates, during the decomposition of ploughed above- and below-ground parts of selected cruciferous species), the use of various plant extracts and the search for sources of resistance in crop plants and wild relatives from the gene bank of the Agricultural Institute of Slovenia.